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. 2021 Jul 15;10:100067. doi: 10.1016/j.ynpai.2021.100067

Fig. 6.

Fig. 6

CCK-BR scFv 77-2 reduces instantaneous firing frequency of TG neurons from FRICT-ION mice. A. Representative current clamp recordings of control and CCK-BR scFv 77-2 treated TG neurons from FRICT-ION mice. Current injections are shown starting from 10 pA sub-rheobase with subsequent 10 pA stepwise current injection. B. Frequency-current (f-I) relationship of control and CCK-BR scFv-treated TG neurons from FRICT-ION mice. This analysis was restricted to neurons that fired 2 or more times with up to 250 pA injections above rheobase. n = 8–9 neurons per condition. CCK-BR scFv-treated neurons displayed significantly reduced firing frequency (p = 0.0024, Mann-Whitney test). CCK-BR scFv (10 ug/ml) was applied to culture media for 1–2 hrs prior to recording. C. Frequency-current (f-I) relationship of CCK-8 (100 nM)-treated TG neurons from FRICT-ION mice in the presence and absence of CCK-BR scFv (10 ug/ml). CCK-8 was applied in vitro at the same time as scFv 77-2 prior to recording. n = 6–11 neurons per condition. The effect of scFv in reducing firing frequency was more significant in the presence of CCK-8 (p < 0.0001, Mann-Whitney test). D. Firing frequency comparison for all conditions based on data in B-C. *p < 0.05, ***p < 0.001, ****p < 0.0001, ANOVA with Tukey’s multiple comparisons test. E. Rheobase was not significantly changed between control and CCK-BR scFv-treated, CCK-8-treated or CCK-8 + CCK-BR scFV-treated (p > 0.05, ANOVA). n = 16–28 neurons per condition. All neurons recorded from were < 30 µm in diameter.