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CCK-BR scFv 77-2 His-tag biomarker brain penetrance. Western blot of the scFv His-tag remaining in the brainstem medullary dorsal horn, trigeminal ganglia (TG), and medial prefrontal cortex (mPFC), 7 weeks after the single i.p. dose was given in male mice with FRICT-ION. The bar graph indicates its brain penetrance with normalized intensity in arbitrary units. n = 4, *p < 0.05; ****p < 0.0001, One-way ANOVA.
