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. 2021 Aug 20;7(34):eabh0250. doi: 10.1126/sciadv.abh0250

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Fig. 2 — Color gradient B-factor analysis of tr-SFX data from blue (24 Å²), to white, to red (36 Å²) for (A) Fe, O₂, substrate/intermediate/product and (B) the protein including α3 (amino acids 47 to 64) and the C-terminal region (α10; amino acids 315 to 331). Note: (i) α3 B-factors rise on O₂ exposure and decrease on IPN formation, (ii) ACV valine–derived atoms become disordered on O₂ exposure but are ordered in the IPN complex, (iii) α10 is involved in ACV binding/IPN product release and remains disordered in IPNS:Fe:IPN complex since IPN is trapped due to lattice packing constraints.