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. 2021 Mar 25;8:610407. doi: 10.3389/fmolb.2021.610407

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Copyright © 2021 Shabalina, Skorova, Chudakova, Anikin, Reshetov, Mynbaev and Petersen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in metabolic activity and area share of single and twin MCTS in the first 36 h of culturing. (A) Resazurin assay was used to assess metabolic activity of the cells. Increase of the fluorescence ratio relative to background values demonstrates an increase in metabolic activity. Data obtained from two experiments with three to nine technical repeats (B) Time-dependent changes in migration zone area shares of the total area of the MCTS. Data obtained from three repeats and presented as the mean ± SD. dynamically change over the time course.