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. 2021 Aug 12;2021:1736819. doi: 10.1155/2021/1736819

Figure 3.

Figure 3

PANTR1 acts as a molecular sponge for miR-587. (a) Biotinylated RNA pull-down assays were conducted by using Bio-NC and Bio-PANTR1 probes. RNA complexes were analyzed by qRT-PCR. (b) AGO2-RIP using anti-AGO2 and anti-IgG antibodies were performed. RNA expressions were detected by qRT-PCR. (c) The binding sites between PANTR1 and miR-587 are shown. (d, e) Luciferase reporter experiments were performed by transfecting NC mimic, miR-587 mimic, reporter vectors harboring PANTR1 wild-type or mutant-type sequences into HepG2 or 293T cells, respectively, and results were statistically analyzed. (f) The miR-587 expression in Sh-NC or Sh-PANTR1-transfected HepG2 or Hep3B was detected by qRT-PCR. Data are shown as the mean ± standard deviation (n = 3). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.