Figure 6.

Identifying a novel lncRNA PANTR1/miR-587/BCL2A1 axis in HCC progression. (a) HepG2 and Hep3B cells were transfected with OE-NC, OE-PANTR1, OE-PANTR1+Sh-NC, and OE-PANTR1+Sh-BCL2A1 as indicated, and transfection efficiencies were detected by qRT-PCR. (b, c) Cell proliferation abilities of pretreated HepG2 and Hep3B cells were detected by CCK-8 assays. (d, e) Flow cytometer assay was applied to evaluate the cell apoptosis rate of pretreated HepG2 and Hep3B cells. (f, g) Transwell migration assays were conducted to evaluate the migration level of pretreated HepG2 and Hep3B cells, and cell migration numbers were accounted. Comparative statistics are shown. Scale bars: 20 μm. (h–j) The Warburg effect phenomenon-related lactate production (h), glycolysis rates (i), and ATP levels (j) in HCC cells were measured. Data are shown as the mean ± standard deviation (n = 3). ^∗P < 0.05 and ^∗∗P < 0.01.