Figure 1.

BBR protects D407 cells from H₂O₂-induced cell apoptosis. (a) D407 cells were treated with different concentrations of H₂O₂ or 0.1% dimethyl sulfoxide (DMSO) (vehicle control) for 24 h, and cell viability was measured by MTT assay. (b) D407 cells were treated with different concentrations of BBR or 0.1% dimethyl sulfoxide (DMSO) (vehicle control) for 24 h, and cell viability was measured by MTT assay (c) D407 cells were pretreated with BBR at indicated concentrations (1 to 6 μM) or 0.1% DMSO (vehicle control) for 2 h and then incubated with or without 100 μM H₂O₂ for further 24 h. Cell viability were measured by MTT assay. (d, e) After pretreatment with 3 μM and 6 μM BBR or 0.1% DMSO (vehicle control) for 2 h, D407 cells were incubated with or without 100 μM H₂O₂ for another 24 h. Apoptotic cells were observed by TUNEL staining (scale bar = 100 μm). (f, g) After pretreatment with 3 μM and 6 μM BBR or 0.1% DMSO (vehicle control) for 2 h, D407 cells were incubated with or without 100 μM H₂O₂ for another 24 h. Apoptotic cells were observed by flow cytometry of PI-Annexin-FITC. The assay was repeated for at least 3 times. ^∗∗∗p < 0.001 versus the control group; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 versus the H₂O₂-treated group were considered significantly different.