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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: J Cell Physiol. 2018 Dec 19;234(8):13220–13232. doi: 10.1002/jcp.27994

FIGURE 7.

FIGURE 7

Knockdown of ERK3 promotes melanoma cell invasion. Invasion assays were performed 2 days posttransfection with siERK3 in (a) BRAFV600E human melanoma cell lines A375 and OCM3 and (b) human WM3211 BRAF WT melanoma cell line. Transwell migration was consistent with transient knockdowns. Knockdown efficiencies were verified by western blots. Migration was quantified by two-chamber transwell migration assays. Migrated cell numbers were quantified from an average of six captured fields (×20 magnification) per insert for two technical replicates per biological replicate (n > 3) after 8–12 hr depending on the cell line. Presented captures were from the replicate with cell number closest to the average. Significance levels (p values) were determined by Student’s t test and expressed as mean ± standard deviation. BRAF: v-Raf murine sarcoma viral oncogene homolog B; ERK3: extracellular signal-regulated kinase 3; siERK3: ERK3 knockdown; WT: wild-type