Figure 5.

Neutrophils-like cells electrotaxis under the effect of fMLP chemoattractant gradient and DC electric field. (A) Quantification of the number of neutrophils migrated per channel toward the cathode and fMLP chemoattractant. The result shows a significant increase (85%-95%) in migration by applying different DC electric field strengths with potentials of 80 mV and 600mV (n=4, p-value<0.001) across the chip. Quantification of the number of neutrophils migrated per channel toward the anode. The result indicates a significantly low, less than ~5-10 cells per channel, directional movement of neutrophils toward the anode. Quantification of the number of neutrophils migrated per channel toward the complete media. Results show no neutrophil-like cells migration toward the complete media due to no electrical or chemical signals. (B) Nikon TiE microscope10X image of the microfluidic device and experiment setup of parallel and synergistic electrotaxis and fMLP chemotaxis. *P ≤ 0.05; ns, not statistically significant.