Table 1.

Time points of sample collection.

Visit		Sample collection (peripheral blood and intestinal tissue)	Treatment	CRP (mg/L)	Calprotectin (μg/g)	PUCAI
February 2015	S0	Presentation with severe acute colitis. Diagnostic endoscopy with biopsies in small intestine and colon.	No treatment	19	2861 μg/g	55
August 2015	S1	Persistent disease activity using infliximab 10 mg/kg every 4 weeks and azathioprine 25 mg/day. Received blood sample.	Infliximab 5 mg/kg/day per 4 weeks, Azathioprine 1dd30 mg, Allopurinol 1dd50 mg, Cholecalciferol 1dd800IE, Omeprazol 1dd10 mg, Cotrimoxazol 1dd30mg	<3	ND	45
December 2016	S2	Subtotal colectomy with ileostoma. Received blood sample and intestinal tissue of patient.	Infliximab 5 mg/kg/day per 4 weeks (last infusion on 21st of December 2015), Azathioprine 1dd30 mg, Allopurinol 1dd50 mg, Cholecalciferol 1dd800IE, Omeprazol 1dd10 mg, Vancomycin 2dd250 mg, Gentamicin 2dd50 mg	209	ND	55
January 2017	S3	Closure of double-barreled ileostomy, one month protectomy and ileoanal pouch anastomosis. No symptoms of intestinal disease. Received blood samples of patient and parents.	No immunosuppressive treatment. No recent antibiotic use.	ND	ND	0
August 2017	S4	Regular visit at outpatient clinic. No symptoms of intestinal disease. Received blood samples of patient and parents.	No immunosuppressive treatment. No recent antibiotic use.	ND	ND	0

CRP C-reactive protein, PUCAI pediatric ulcerative colitis activity index, dd daily dosis, ND not determined.