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. 2021 May 11;14(5):1183–1202. doi: 10.1038/s41385-021-00407-5

Fig. 9. IL-17 induces chemokines and cytokines necessary for neutrophil recruitment.

Fig. 9

WT and Il17A−/− mice were aerosol infected with B. pertussis. Prior to and 2 h, 7 and 17 d post challenge mice were sacrificed, and mRNA was extracted from nasal tissue for RT-PCR analysis. a Gene expression of Cxcl1, Cxcl2, Cfs3 and IL-1β in nasal tissue of WT and Il17A−/− mice was determined by RT-PCR and normalized to 18S RNA. b WT and Il17A−/− mice were aerosol infected with B. pertussis. Up to day 60 post challenge nasal washes and lung homogenates were prepared and protein concentration of CXCL1 was determined by ELISA. c Convalescent WT and Il17A−/− were aerosol infected with B. pertussis. On day 3, 7 and 14 post re-challenge nasal washes and lung homogenates were prepared and protein concentration of CXCL1 was determined by ELISA. d C57BL/6 mice were treated intranasally with IL-17A and euthanized 2 h later. CXCL1 concentration in nasal washes was determined by ELISA and Cxcl1 gene expression was analyzed by RT-PCR and normalized to 18S RNA. Statistical analysis: Two-way ANOVA followed by Sidak’s post-test, d unpaired, two-tailed t-test, *p < 0.05, **p < 0.01, ***p < 0.001; a–c n = 4/ group; d n = 8/group, pooled data from two independent experiments, mean ± SEM.