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. 2021 Aug 20;11:16944. doi: 10.1038/s41598-021-96258-y

Figure 1.

Figure 1

PA gel embedding does not preserve sub-30 nm structure. (a) DNA origami labeling strategy: Selected staple strands contain an acrydite group for PA gel anchoring and can later be fluorescently labeled through hybridization of a secondary oligo featuring an internal Cy5 dye. (b) Linear DNA origami design featuring 10 labeling sites spaced 28 nm apart. (c–e) STORM image analysis for linear origami (c) without gel (N = 100 origami objects), (d) post-embedding in a PA gel without denaturation (N = 100 origami objects), and (e) post-embedding in a PA gel (N = 98 origami objects). Insets show representative STORM images of single origami objects. The scale bars in the insets are 100 nm. Histograms show nearest neighbor emitter distances within origami objects. Red curves show Gaussian fits of the first peaks, mean ± standard deviation: (c) 29.1 ± 8.3 nm and (d) 29.4 ± 8.3 nm.