Skip to main content
. 2021 Aug 20;4:993. doi: 10.1038/s42003-021-02502-6

Fig. 2. Mammary epithelial proliferation is significantly higher in younger lambs than in those approaching puberty.

Fig. 2

ac IHC for Ki67 in mammary gland from lambs < 2 mo (a) and 5–9.5 mo (b) and accompanying mask derived using an algorithm detecting intra-epithelial Ki67 positive events (c). d Scatter plot demonstrating that the distributions of epithelial nuclear Ki67 positivity differ significantly between younger and older lambs. Dots represent individual lambs. Bars represent mean ± standard deviation. * p < 0.05. e, f IF for Ki67 (gold), α-SMA (cyan) and DNA (DAPI; magenta) demonstrating that the majority of Ki67 positive nuclei are in the luminal epithelial layer (arrowheads), with rare Ki67 positive nuclei in myoepithelial cells (arrow). e 1 do lamb. f 9.5 mo lamb. do, days old; mo, months old. Images are representative of eight (a), four (b), twelve (c) and three (e, f) biological repeats. All IHC images have haematoxylin counterstain. Scale bar = 200 μm (ac); 100 μm (e); 50 μm (f).