Table 1.
Antibodies employed for immunohistochemistry, immunofluorescence, and CUBIC.
| Target | Application (IHC, immunohistochemistry; IF, immunofluorescence; CUBIC, 3D tissue clearing) | Species and clone | Dilution | Manufacturer | Catalogue number |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| Alpha smooth muscle actin | IF; dual colour IHC | Rabbit monoclonal [EPR5368] | 1:2000 | Abcam | Ab124964 |
| Alpha smooth muscle actin | CUBIC; dual colour IHC | Mouse monoclonal anti-human 1A4 | 1:100 (CUBIC) 1:400 (dual colour IHC) | Dako/Agilent | M0851 |
| CD3 | Dual colour IHC | Mouse monoclonal anti-human clone F7.2.38 | 1:250 | Dako/Agilent | M7254 |
| CD20 | Dual colour IHC | Rabbit polyclonal | 1:800 | Thermo Fisher Scientific | RB-9013-P1 |
| E-cadherin | Dual colour IHC | Rabbit monoclonal | 1:400 | Cell Signalling Technology | #3195 |
| IBA1 | IHC; dual colour IHC | Mouse monoclonal, clone 20A12.1 | 1:800 | Millipore | MABN92 |
| IBA1 | CUBIC | Rabbit monoclonal [EPR16588] | 1:400 | Abcam | Ab178846 |
| Ki67 | IHC; IF | Mouse monoclonal anti-human clone MIB-1 | 1:100 | Dako/Agilent | M7240 |
| PNAd | IHC | Rat monoclonal anti-mouse/ human clone MECA-79 | 1:100 | BioLegend | 120802 |
| Secondary antibodies | |||||
| Mouse IgG, Alexa Fluor Plus 488 | IF | Goat | 1:500 | Thermo Fisher Scientific | A32723 |
| Mouse IgG, Alexa Fluor 568 | IF | Goat | 1:500 | Thermo Fisher Scientific | A11031 |
| Rabbit IgG, Alexa Fluor Plus 488 | CUBIC | Goat | 1:500 | Thermo Fisher Scientific | A32731 |
| Rabbit IgG, Alexa Fluor Plus 647 | CUBIC | Goat | 1:500 | Thermo Fisher Scientific | A32733 |
| Rat IgG, peroxidase labelled | IHC | Goat | 1:400 | Vector laboratories | PI-9400 |