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. 2021 Aug 20;11:16830. doi: 10.1038/s41598-021-94286-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Concentration of eDNA in (a) tank water and (b) sediment after the introduction of fish. Nine samples (triplicates from each of three tanks, indicated by circles) for test groups and three samples (triplicates from one tank, triangles) for control were analyzed and plotted. Asterisks indicate significant differences between two consecutive sampling days (p < 0.05, Tukey’s HSD test). The best models shown in Supplementary Table S3 were fitted.