Subject	Agricultural and Biological Sciences (General)
Specific subject area	Effects of soil microplastics and watering regime on the behaviour of fungus-gnat females
Type of data	Table Image Figure
How data were acquired	J-Watcher, olfactometer, DNA extraction, DNA barcoding, SANGER sequencing; ImageJ
Data format	Raw Analyzed
Parameters for data collection	The behaviour (% residence time) of fungus gnat mated females was evaluated for 10 min in a multiple one-way olfactometer device at 25 °C. Odour sources (i.e., single pots of a plant-soil system, a fungus colony or clean air) were placed in glass chambers sealed with parafilm to a Teflon disk. A humified airflow conveyed the volatiles from the chamber to each olfactometer. DNA from insect tissues was amplified with universal primers for Sanger sequencing. For species identification, Sanger sequences were compared to sequences deposited in GenBank using BLAST.
Description of data collection	J-Watcher recordings of residence time data; ImageJ for description of plastic particle sizes. DNA barcoding (Sanger sequencing) for insect identification.
Data source location	Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università degli Studi di Perugia, Perugia, Italy
Data accessibility	With the article plus DNA sequence in NCBI (accession numbers: MW947257-MW947259 available at https://www.ncbi.nlm.nih.gov/nuccore?term=((MW947257.1%5BAccession%5D)%20OR%20MW947258.1%5BAccession%5D)%20OR%20MW947259.1)
Related research article	[1] Rondoni, G., Chierici, E., Agnelli, A., Conti, E., Microplastics alter behavioural responses of an insect herbivore to a plant-soil system, Science of the Total Environment, 787: 147716. Doi: 10.1016/j.scitotenv.2021.147716