Fig. 5.

The functional regions of 2B, 2C, 3C^pro, and L^pro responsible for reduction of RIP2. A The experiment was performed as “materials and methods” described. B Schematic representation showing a series of FLAG-tagged truncated 2B and 2C mutants. C, D, E, F PK-15 cells cultured in six-well plates were transfected with 1.5 μg FLAG-2B-, FLAG-2C-, FLAG-3C-, FLAG-L-, or their mutant expressing plasmids for 24 h. The cells were collected and analyzed by Western blotting.