Fig. 5.

The expression of seven known piRNAs in HRV16-infected H1-HeLa cells at 12 h, 24 h and 36 h p.i. was validated by RT-qPCR. The infected and normal cells of three repetitive wells were collected at 12 h, 24 h, and 36 h post-infection. Total cellular RNA of each well was isolated with TRIzol Reagent. cDNAs of piRNAs were gotten using stem-loop RT primers. RT-qPCR was performed using specific forward primer of each piRNA and a universal reverse primer. U6 was used as an endogenous control for data normalization. The relative amount of expressed mRNA was calculated by the log2 conversion of 2^−△△Ct.