Table 3.

Primer sequences for real-time RT-qPCR.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Products length, bp	Tm, °C	E-value, %
ACC1	AATCAACATCCGCCTGACTCCAAC	CCTGCTTGTCTCCGTATGCTTGG	176	59.0	90.2
ASS	CCCAGGAGGCACAATTCTGT	CGCACAAAATCACACTCCGG	153	57.6	93.8
ATGL	CTTCCTCTCCGCAACAAGTC	TGGTGCTGTCTGGAGTGTTC	211	55.8	100.0
Caspase-3	ATCACAGCAACTACGCCTCATTCG	GCCTCTGCAAGCCTGGATGAAG	176	61.6	98.9
Caspase-8	AAGACGCATCTGTTCGCTTCCTG	GCGACAGCTTCAGCCTATCCATC	116	61.6	98.9
Caspase-9	TGCGGAGGAGGTGAACGAGAC	CGGTTCGTCGGACATGCTCAG	138	62.8	90.5
C/EBPα	CACGGACAACGACAGACTGA	GCCACACACCAACTCACGTA	213	60.5	93.4
C/EBPβ	TCTGATTCCAGGGTGTCCTC	TCATCTGCTCAGCCACTCTG	181	57.1	98.8
EF1α	AATCGGCGGTATTGGAACTG	TCCACGACGGATTTCCTTGA	205	58.5	102.0
FASN	AGGCATTGTGGAGGGTGTAG	CCAGTCCACCAGTGATGATG	233	56.8	97.1
HMGCR	GGAAGAGGAAGAGGACAACAAGCC	GAACCATGACCAGGCCAAGCG	80	56.6	101.0
HSL	TGATGTTTGCCAAGAAGCTG	CTGATGGACTGGTGCTCTGA	228	57.8	93.8
Leptin	TGCAACTTTTAAGTGGGGGTA	TGTTGTAACCCTCCAGCACGG	201	59.0	103.5
IL1β	CTGAACATCAAGGGCACAGA	GTTGAAGGGGACAGACCTGA	192	60.8	92.8
IL8	GAGCTGATTCCTGCCAACTC	CCGATCTGTTCAGGGTGTTC	153	55.8	98.0
LPL	AGCACCTCCAAAACCTTCCT	TCTGAGCTGCCACCACATAG	169	58.8	92.9
PPARγ	AGGCCTGCTGAATGTGAAGC	GCTGGATGAAGTGGACGTGG	170	58.0	93.3
TNF-α	GACTCCATAGGCAGCAAAGC	AGAAAGTCTTGCCCTCGTCA	205	60.8	103.2

ACC1 = acetyl-CoA carboxylase 1; ASS = argininosuccinate synthetase; ATGL = adipose triglyceride lipase; Caspase = cysteine-aspartic proteas; C/EBP = CCAAT-enhancer-binding protein; EF1α = eukaryotic translation elongation factor 1 alpha; FASN = fatty acid synthase; HMGCR = 3-hydroxy-3-methylglutaryl-CoA reductase; HSL = hormone-sensitive triglyceride lipase; IL = interleukin; LPL = lipoprotein lipase; PPAR = peroxisome proliferator activated receptor; TNF = tumor necrosis factor.