Fig. 6.

Western blotting was performed to validate the MS results.A, serum from additional 12 patients with TNBC and 12 healthy donors was independently assessed by Western blotting. A Coomassie blue–stained gel containing total proteins was used as a loading control. B, the relative gene expression levels were determined separately for each treatment as the mean ± SEM (n = 6), ∗p < 0.05. C, validation of selected proteins indicated that the results from MS generally agreed well with the Western blotting results. ns, not significant; TNBC, triple-negative breast cancer.