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. 2021 Jul 13;20:100121. doi: 10.1016/j.mcpro.2021.100121

Fig. 7.

Fig. 7

Expression of CD151 in exosomes derived from patients with TNBC and TNBC cell lines.A, top 20 upregulated proteins identified in TNBC patient-derived exosomes ranked by p value. Significance of their upregulation in TNBC patient–derived exosomes is listed accordingly. B, immunoblot was performed to access the expression levels of exosomal CD151 expression in additional patients with TNBC and healthy donors. “30 to 37” and “23 to 30” represent the individual healthy donors and patients with TNBC. Flotillin-1 was used as a loading control. C, the relative quantified results of the immunoblots are represented as mean ± SEM (n = 4). ∗p < 0.05. D, immunoblot analysis of CD151 expression in WCLs of breast cancer cell lines (non-TNBC cell lines: T47D and MCF7; TNBC cell lines: MDA-MB-468, BT549, and MDA-MB-231). Total protein (15 μg) was loaded onto the gels. β-actin was used as a control for equal loading. E, immunoblot analysis of CD151 expression in exosomes derived from breast cancer cell lines. Total protein (15 μg) was loaded onto the gels. Flotillin-1 was used as a control for equal loading. ns, not significant; TNBC, triple-negative breast cancer; WCLs, whole-cell lysates.