Fig. 1.

Charactrization of hDPSCs. (A) After pulp tissue culture for 3 days, spindle-shaped cells spread out in the culture flask. P3 generation cells were spindle-shaped. Mineralized nodules and lipid droplets were found when hDPSCs were cultured in osteogenic or adipogenic medium respectively. (B) Human dental pulp stem cells (hDPSCs) were positive for Vimentin、STRO-1、C-Kit and Nestin and negative for CK14. (C) Flow cytometric analyses showed that hDPSCs were positive for CD73 and CD90 and negative for CD3, CD31 and CD34. The experiments were performed in triplicates.