Fig. 3.
Bone-targeting capability of GPSP. (a) SEM images of hydroxyapatite tablets incubated with distilled water, saporin, GPS and GPSP, respectively for 24 h. The white arrows indicate GPS or GPSP nanoparticles adsorbed on the hydroxyapatite tablets. (b) Fluorescence images of hydroxyapatite tablets incubated with saporinCy5.5, GPSCy5.5 and GPSCy5.5P, respectively for 24 h. (c) Relative radiant efficiency of the samples in (b) measured by IVIS. (d) Fluorescence images of tibias from healthy mice and bone tumor-bearing mice incubated with GPSCy5.5 and GPSCy5.5P, respectively for 24 h. (e) Relative radiant efficiency of the samples in (d). (f) Fluorescence images of mice intravenously injected with GPSCy5.5 and GPSCy5.5P for 3, 6, 12, 24, 36 and 48 h, respectively. The red dotted circles represent the tumor sites. (g) Fluorescence images of the main organs and tissues collected from mice after treatment with GPSCy5.5 and GPSCy5.5P, respectively for 24 h. (h) Quantitative analysis of saporinCy5.5 in the main organs at 24 h post-injection. He = heart; Li = liver; Sp = spleen; Ki = kidney; Lu = lung; Tu = tumor; Ti = tibia. *p < 0.05 and **p < 0.01 analyzed by student's t-test, one tailed. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)