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. 2021 Aug 5;19:4412–4425. doi: 10.1016/j.csbj.2021.08.002

Fig. 5.

Fig. 5

Analysis of the hypersensitivity phenotype caused by Ato1-GFP E144A mutation. A – Spot assay of S. cerevisiae IMX1000 transformed with Ato1-GFP mutant alleles in the residues E144, L219 and E144/L219, grown on YNB media containing 1%, 0.5% and 0.1% of lactic acid, pH 5.0 (LA) at 18 °C for 10 days. B – Spot assay of S. cerevisiae IMX1000 transformed with Ato1-GFP mutant alleles in the residues E144A, L219A, E144/L219A and F98A/E144A, grown on YNB containing 0.05%, 0.1%, 0.5% and 2.0% succinic acid, pH 5.0 (SA) at 18 °C for 10 days. C – Spot assay of S. cerevisiae CEN.PK 113-5D transformed with Ato1-GFP mutants in the residues E144, L219, E144/L219, grown on YNB media containing acetic acid (0.5%, pH 6.0) (AA) or lactic acid (0.5%, pH 5.0) (LA) at 18 °C for 10 days.