Table 2.
List of primers used in this study.
| Name | Sequence |
|---|---|
| K86A fw | CCTGCTCCAGTGCACGCTTTTGCTAATCCTGCGC |
| K86A rev | GCGCAGGATTAGCAAAAGCGTGCACTGGAGCAGG |
| F98A fw | CTTAGGTCTTTCAGCCGCCGCGTTGACGACATTTG |
| F98A rev | CAAATGTCGTCAACGCGGCGGCTGAAAGACCTAAG |
| Q133A fw | GTGGTTTGGTGGCTTTGATTGCTGGTATTTG |
| Q133A rev | CAAATACCAGCAATCAAAGCCACCAAACCAC |
| E140A fw | CTGGTATTTGGGCTATAGCTTTGGAAAATAC |
| E140 rev | GTATTTTCCAAAGCTATAGCCCAAATACCAG |
| E144A fw | GAGATAGCTTTGGCTAATACTTTTGGTGGTAC |
| E144A rev | GTACCACCAAAAGTATTAGCCAAAGCTATCTC |
| N145A fw | GAGATAGCTTTGGAAGCTACTTTTGGTGGTAC |
| N145A rev | GTACCACCAAAAGTAGCTTCCAAAGCTATCTC |
| T146A fw | GATAGCTTTGGAAAATGCTTTTGGTGGTACCG |
| T146A rev | CGGTACCACCAAAAGCATTTTCCAAAGCTATC |
| F147A fw | TTTGGAAAATACTGCTGGTGGTACCGCATTA |
| F147A rev | TAATGCGGTACCACCAGCAGTATTTTCCAAA |
| T150A fw | AATACTTTTGGTGGTGCTGCATTATGTTCTTA |
| T150 rev | TAAGAACATAATGCAGCACCACCAAAAGTATT |
| L152A fw | TTGGTGGTACCGCAGCTTGTTCTTACGGTG |
| L152A rev | CACCGTAAGAACAAGCTGCGGTACCACCAA |
| C153A fw | GGTGGTACCGCATTAGCTTCTTACGGTGGGTTTTG |
| C153A rev | CAAAACCCACCGTAAGAAGCTAATGCGGTACCACC |
| Y155A fw | CATTATGTTCTGCTGGTGGGTTTTGGTTGAG |
| Y155A rev | CTCAACCAAAACCCACCAGCAGAACATAATG |
| Y155S fw | CATTATGTTCTTCTGGTGGGTTTTGGTTGAG |
| Y155S rev | CTCAACCAAAACCCACCAGAAGAACATAATG |
| Y155F fw | CATTATGTTCTTTCGGTGGGTTTTGGTTGAG |
| Y155F rev | CTCAACCAAAACCCACCGAAAGAACATAATG |
| F158A fw | GTTCTTACGGTGGGGCTTGGTTGAGTTTCGCTG |
| F158A rev | CAGCGAAACTCAACCAAGCCCCACCGTAAGAAC |
| W159A fw | CTTACGGTGGGTTTGCTTTGAGTTTCGCTGC |
| W159A rev | GCAGCGAAACTCAAAGCAAACCCACCGTAAG |
| E174A fw | GTTTGGTATCTTGGCTGCTTACGAAGACAATG |
| E174A rev | CATTGTCTTCGTAAGCAGCCAAGATACCAAAC |
| E177A fw | TCTTGGAAGCTTACGCTGACAATGAATCTG |
| E177A rev | CAGATTCATTGTCAGCGTAAGCTTCCAAGA |
| D178A fw | GGAAGCTTACGAAGCTAATGAATCTGATTTG |
| D178A rev | CAAATCAGATTCATTAGCTTCGTAAGCTTCC |
| E180A fw | CTTACGAAGACAATGCTTCTGATTTGAATAATG |
| E180A rev | CATTATTCAAATCAGAAGCATTGTCTTCGTAAG |
| D182A fw | GAAGACAATGAATCTGCTTTGAATAATGCTTTAG |
| D182A rev | CTAAAGCATTATTCAAAGCAGATTCATTGTCTTC |
| L191A fw | GCTTTAGGATTTTATGCTTTGGGGTGGGCCATC |
| L191A rev | GATGGCCCACCCCAAAGCATAAAATCCTAAAGC |
| W194A fw | TTATTTGTTGGGGGCTGCCATCTTTACGTTTG |
| W194A rev | CAAACGTAAAGATGGCAGCCCCCAACAAATAA |
| F197A fw | GTTGGGGTGGGCCATCGCTACGTTTGGTTTAAC |
| F197A rev | GTTAAACCAAACGTAGCGATGGCCCACCCCAAC |
| T198A fw | GGGTGGGCCATCTTTGCTTTTGGTTTAACCG |
| T198A rev | CGGTTAAACCAAAAGCAAAGATGGCCCACCC |
| K207A fw | CCGTTTGTACCATGGCTTCCACTGTTATGTTCTTTTTG |
| K207 rev | CAAAAAGAACATAACAGTGGAAGCCATGGTACAAACGG |
| F216A fw | GTTATGTTCTTTTTGTTGGCTTTCTTACTAGCATTAAC |
| F216A rev | GTTAATGCTAGTAAGAAAGCCAACAAAAAGAACATAAC |
| L219A fw | GTTGTTCTTCTTAGCTGCATTAACTTTCCTACTG |
| L219A rev | CAGTAGGAAAGTTAATGCAGCTAAGAAGAACAAC |
| L219V fw | GTTGTTCTTCTTAGTTGCATTAACTTTCCTACTG |
| L219V rev | CAGTAGGAAAGTTAATGCAACTAAGAAGAACAAC |
| L219G fw | GTTGTTCTTCTTAGGTGCATTAACTTTCCTACTG |
| L219G rev | CAGTAGGAAAGTTAATGCACCTAAGAAGAACAAC |
| L219S fw | GTTGTTCTTCTTATCTGCATTAACTTTCCTACTG |
| L219S rev | CAGTAGGAAAGTTAATGCAGATAAGAAGAACAAC |
| L219P fw | GTTGTTCTTCTTACCAGCATTAACTTTCCTACTG |
| L219P rev | CAGTAGGAAAGTTAATGCTGGTAAGAAGAACAAC |
| L219W fw | GTTGTTCTTCTTATGGGCATTAACTTTCCTACTG |
| L219W rev | CAGTAGGAAAGTTAATGCCCATAAGAAGAACAAC |
| F223A fw | CTTACTAGCATTAACTGCTCTACTGTTGTCTATTGG |
| F223A rev | CCAATAGACAACAGTAGAGCAGTTAATGCTAGTAAG |
| L226A fw | CATTAACTTTCCTACTGGCTTCTATTGGTCACTTTG |
| L226A rev | CAAAGTGACCAATAGAAGCCAGTAGGAAAGTTAATG |
| H230A fw | CTGTTGTCTATTGGTGCTTTTGCTAATAGACTTGG |
| H230 rev | CCAAGTCTATTAGCAAAAGCACCAATAGACAACAG |
| R239A fw | GACTTGGTGTCACAGCTGCTGGTGGTGTCCTGG |
| R239A rev | CCAGGACACCACCAGCAGCTGTGACACCAAGTC |
| Y254A fw | CTTTCATTGCTTGGGCTAACGCATATGCAGG |
| Y254A rev | CCTGCATATGCGTTAGCCCAAGCAATGAAAG |
| V260A fw | CGCATATGCAGGTGTCGCTACAAAGCAGAA |
| V260 rev | TTCTGCTTTGTAGCGACACCTGCATATGCG |
| T262A fw | CGCATATGCAGGTGTTGCTGCTAAGCAGAATTC |
| T262A rev | GAATTCTGCTTAGCAGCAACACCTGCATATGCG |
| K263A fw | GCAGGTGTTGCTACAGCTCAGAATTCATATGTACTG |
| K263A rev | CAGTACATATGAATTCTGAGCTGTAGCAACACCTGC |
| SatP_resc fw | AAAAGGATCCATGGGTAACACCAAATTAGCC |
| SatP_resc rev | TTTTCTCGAGCTAATGACTTTCACCGATAGGCAAG |
| L131A fw | CGTTTTCTTTTCTGCTACAGTTTTGTTCGCTTTG |
| L131A rev | CAAAGCGAACAAAACTGTAGCAGAAAAGAAAACG |