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. 2021 Aug 16;13:6451–6471. doi: 10.2147/CMAR.S322179

Figure 12.

Figure 12

Silencing of SNHG4 inhibits proliferation, migration and invasion in vitro. qRT-PCR to detect the relative silencing levels of SNHG4 in Huh7 and MHCC-LM3 cells (A and B). Representative images of EdU assay of Huh7 and MHCC-LM3 with si-NC, si-SNHG4 and wild-type cells (C and D). Colony formation ability of Huh7 and MHCC-LM3 transfected with si-NC and si-SNHG4 and wild-type cells (E and F). Transwell assay for investigating migration and invasive properties Huh7 and MHCC-LM3 transfected with si-NC, si-SNHG4 and wild-type cells (G and H). Bcl-2, Bax, Cleaved casp-3, Cleaved casp-9, Ki67 and PCNA were assessed by Western blot analysis (I and J). **P<0.01, ***P<0.001, n=3.