Investigation of ROS detection using the RNO bleaching (RNO-imidazole) method. Normalized absorption curves of RNO at 440 nm in the presence of (a) DI water, TPEPy-I, or TPEPy-PF6 under 10 W of MW irradiation, (b) DI water or TPEPy-I under 2 and 10 W of MW irradiation, (c) TPEPy-I (5 and 10 μM) under 2 and 10 W of MW irradiation, and (d) DI water, BQ, NaN3, TPEPy-I, TPEPy-I + NaN3, or TPEPy-I + BQ under 10 W of MW irradiation.