Figure 4.

The differentiation of visceral white adipocytes was attenuated in Ext1^Δ/WTmice of epididymal white adipose tissue.A and B, relative mRNA expression levels of differentiation markers in vWAT. n = 5–11. C and D, left, representative images of Western blot in Pparγ. Right, relative protein level of Pparγ in vWAT. n = 3. The mean Pparγ expression level in control vWAT was set to 1. E, left, observation of vWAT by H&E staining. The white scale bar represents 50 μm. Right, the averaged area of adipocytes in upper pictures. n = 9–11. At least 100 areas were measured, per mouse. F and G, relative mRNA expression levels of adipogenic genes in vWAT. n = 3–18. In A, B, F, and G, the mean mRNA expression level of control vWAT was set to 1. H, plasma triglyceride concentration 6 h after starvation. n = 6–9. Gray: control mice fed with ND; red: Ext1^Δ/WT mice fed with ND; blue: control mice fed with HFD; and green: Ext1^Δ/WT mice fed with HFD. ∗Comparison of mice fed ND; ^#Comparison of mice fed HFD. ∗ and ^#p < 0.05; ∗∗ and ^##p < 0.01. Acaca, acetyl-CoA carboxylase alpha; Acacb, acetyl-CoA carboxylase beta; Cebpδ, CCAAT/enhancer-binding protein δ; Cebpβ, CCAAT/enhancer-binding protein β; HFD, high-fat diet; Klf15, krüppel-like factor 15; ND, normal diet; Pparγ, peroxisome proliferator–activated receptor gamma; Slc27a1, long-chain fatty acid transport protein 1; Srebp1, sterol regulatory element–binding protein 1; vWAT, visceral white adipose tissue.