Figure 5.

Expressions of BMP4–FGF1 signaling molecules were altered in vWAT of Ext1^Δ/WTmice.A and C, relative mRNA expression levels of BMP4–FGF1 pathway–related genes in vWAT. n = 5–18. The mean mRNA expression level of control vWAT was set to 1. B and D, left, representative images of Western blot in Fgfr1. Right, relative protein level of Fgfr1 in vWAT. n = 3. The mean Fgfr1 expression level of control WAT was set to 1. We intentionally showed the same Western blot images of Actb for Figures 4C and 5B because we used same adipose tissues for Western blotting in these experiments. We also showed the same Actb images for Figures 4D and 5D because we used same adipose tissue homogenates to examine the expression levels of Pparγ (Fig. 4D) and Fgfr1 (Fig. 5D). Gray: vWAT isolated from control mice fed with ND; red: vWAT isolated from Ext1^Δ/WT mice fed with NDl; blue: vWAT isolated from control mice fed with HFD; and green: vWAT isolated from Ext1^Δ/WT mice fed with HFD. ∗Comparison of mice fed with ND; ^#Comparison of mice fed with HFD. ∗ and ^#p < 0.05; ^##p < 0.01. BMP4, bone morphogenetic protein 4; FGF1, fibroblast growth factor 1; Fgfr1, fibroblast growth factor receptor 1; HFD, high-fat diet; ND, normal diet; Pparγ, peroxisome proliferator–activated receptor gamma; vWAT, visceral white adipose tissue.