Table 1.
Mode | T2 (ms) |
Area of T2 (%) |
|||||
---|---|---|---|---|---|---|---|
T21 | T22 | T23 | A21 | A22 | A23 | ||
Pretreatment | Control | 0.43 ± 0.05c | 265.61 ± 5.01a | 1072.27 ± 3.35b | 2.19 ± 0.10a | 39.26 ± 0.20d | 58.55 ± 0.51a |
SM-20 | 1.75 ± 0.09b | 256.23 ± 4.20b | 925.26 ± 5.02e | 0.91 ± 0.07b | 48.78 ± 0.21b | 50.31 ± 0.32c | |
SM-40 | 10.11 ± 0.10a | 231.01 ± 4.51c | 936.60 ± 3.71d | 0.72 ± 0.08c | 58.90 ± 0.15a | 40.38 ± 0.15e | |
SeDM | — | 200.92 ± 4.24d | 1232.85 ± 6.02a | — | 57.79 ± 1.65a | 42.21 ± 0.25d | |
SiDM | — | 206.02 ± 3.62d | 1065.07 ± 7.65c | — | 46.71 ± 0.22c | 53.29 ± 0.51b | |
Vacuum freeze drying | Control | 0.43 ± 0.05a | 7.06 ± 0.13a | 75.65 ± 0.15a | 29.33 ± 0.15c | 39.62 ± 0.14b | 31.05 ± 0.50a |
SM-20 | 0.22 ± 0.02bc | 6.14 ± 0.15c | 68.27 ± 0.21c | 32.24 ± 1.07b | 40.08 ± 0.45b | 27.68 ± 0.33c | |
SM-40 | 0.38 ± 0.08a | 6.77 ± 0.20b | 70.83 ± 0.12b | 30.70 ± 0.25bc | 39.83 ± 0.18b | 29.47 ± 0.18b | |
SeDM | 0.14 ± 0.04c | 4.64 ± 0.15d | 49.77 ± 0.15e | 42.33 ± 1.12a | 38.55 ± 0.31c | 19.12 ± 0.14d | |
SiDM | 0.25 ± 0.05b | 4.82 ± 0.21d | 65.79 ± 0.12d | 24.33 ± 0.70d | 48.17 ± 0.20a | 27.50 ± 0.29c |
Note: Values are means ± SD, n ≥ 3. Different letters in the same column mean significant difference at p < 0.05. T23 refers to the the free water’s transverse relaxation time; T21 and T22 refer to the transverse relaxation time of the bound water and the immobilized water, respectively. A21 represents the corresponding water fraction to T21, A22 represents the corresponding water fraction to T22, and A23 represents the corresponding water fraction to T23. Control: no-ultrasound; SM-20: single-frequency mode of 20 kHz; SM-40: single-frequency mode of 40 kHz; SeDM: sequential dual-frequency mode of 20/40 kHz; SiDM: simultaneous dual-frequency mode of 20 and 40 kHz.