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. 2021 Aug 12;15:100381. doi: 10.1016/j.ynstr.2021.100381

Fig. 3.

Fig. 3

Analysis of dendritic morphology and miR-9-5p expression in primary hippocampal cultures treated with corticosterone and ketamine. (A) Timing of primary hippocampal cultures in vitro treatment with CORT and KET. (B) Representative image of a hippocampal neuron used for morphological study and reconstruction of the dendritic tree performed using plug-in Fiji at T0 (blue line) and T1 (red line). (C) The difference between T1 and T0 measurements within the same cell is reported as Δ dendritic length T1-T0 (μm), for vehicle, CORT and CORT plus KET treatment. Tukey's post-hoc test: *p < 0.05 vs DMSO, #p < 0.05 vs CORT; n = 10 (DMSO), n = 22 (CORT), n = 10 (CORT + KET). (D) miR-9-5p expression in primary hippocampal neurons treated with CORT and KET. Tukey's post-hoc test: **p < 0.01 vs DMSO, #p < 0.05 vs CORT; n = 9–10. Scale bar 50 μm.. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)