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. 2021 Jul 21;297(3):100993. doi: 10.1016/j.jbc.2021.100993

Figure 5.

Figure 5

Transduction with PGRN and L-PGRN does not alter the cellular composition of primary cortical cultures.A, primary neuronal cultures were transduced with vectors expressing GFP, PGRN, or L-PGRN under the PGK promoter shortly after plating, then analyzed at DIV14. B and C, cultures were immunostained for NeuN and GFAP to identify neurons and astrocytes, and for MAP2 to assess neuronal dendritic outgrowth. D–F, no significant differences in any of these markers were observed in cultures transduced with each vector (n = 15–16 per group). Scale bars in B and C represent 100 μm.