Fig. 4.

Examination of nuclear Sirt1 activity, NAD⁺/NADH levels, nuclear NAD⁺ consumption, NAD⁺ metabolites, NAD⁺ salvage pathway enzymes and ATP levels in the LV tissue of 10 week WT and KO littermates. (A) Nuclear Sirt1 activity. (B) NAD⁺ and NADH levels, and NAD⁺:NADH ratio. (C) nuclear NAD⁺ consumption. (D) NAM levels. (E) NMN levels. (F) Nampt and Nmnat1 protein expression. (G) ATP levels. All studies were performed in 10 week KO mice versus WT littermates. Data are mean ± SEM (n = 4–8 mice/genotype). Unpaired two-tailed Student's t-tests were used. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus WT littermates.