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. 2021 Jun 10;46:102038. doi: 10.1016/j.redox.2021.102038

Fig. 5.

Fig. 5

Protein markers of mitochondrial fusion and fission, and mitochondrial dysfunction in the LV tissue of 4 and 10 week WT and KO littermates. (A) Representative immunoblots and densitometry showing total protein expression of mitochondrial fusion markers: Mfn and Opa1, and mitochondrial fission markers: Drp1, Fis1, and Mff. (B) Representative immunoblots and densitometry of the mitochondrial fraction assessing the markers of mitochondrial dynamics in (A). (C) Representative immunoblots and densitometry showing total protein expression of Pink1 and Parkin. (D) Representative immunoblots and densitometry of the mitochondrial fraction assessing Pink1 and Parkin expression. Gapdh was used as a loading control for total protein-loading in (A, C), while Hsp90 and Tomm20 were used as protein-loading controls for cytoplasmic and mitochondrial fractions in (B, D), respectively. Data are mean ± SEM (n = 4–6 mice/genotype/age). Unpaired two-tailed Student's t-tests were used. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus WT. a.u., arbitrary unit. n.s., not significant.