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. 2021 Jul 26;53:101311. doi: 10.1016/j.molmet.2021.101311

Figure 1.

Figure 1

Glucokinase is expressed in tanycytes facing the ventromedial arcuate nucleus. A-C. Representative images of Gck mRNA expression (cyan dots) along the third ventricle (arrows in A and C) and in the ARH parenchyma (asterisk in A) in adult male mice. D-E. Representative images of Gck promoter activity (mCherry, red) along the third ventricle (arrows) and in parenchymal cells (arrowheads) in adult male mice. F–H. Representative images of GCK immunostaining (cyan) along the ventricle (arrows). GCK staining colocalizes with vimentin staining (red in G and H). I. Schematic representation of Gck expression (cyan) along the third ventricle on the anteroposterior axis, based on in situ hybridization and immunohistochemistry analysis. J-L. Representative images of tanycyte (tdTomato, red)/NPY neuron (green) units in the vmARH. Tanycytes contact NPY neurons (arrowheads in K and L). GCK (cyan) is expressed in tanycytes (arrows) and NPY neurons (empty arrows). ARH, arcuate nucleus; DMH, dorsomedial nucleus; GCK, glucokinase; ME, median eminence; NPY, neuropeptide Y; Vim, vimentin; vmARH, ventromedial arcuate nucleus; VMH, ventromedial nucleus; 3V, third ventricle. Cell nuclei are counterstained using DAPI (white in B, C, E, G, H). Scale bars: 50 μm in A, D, F, and 20 μm in J. Refer Supplementary Figure 1.