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. 2021 Jul 26;53:101311. doi: 10.1016/j.molmet.2021.101311

Figure 4.

Figure 4

Tanycyte ablation increases adiposity. A-B. Bodyweight (A) and changes in bodyweight (compared to preinjection bodyweight) (B) in adult male TanGckf/f and TanGck−/− mice (2 cohorts, n = 16/19, respectively). C-E. Changes in bodyweight (C), fat mass ratio (D), and lean mass ratio (E) in adult male TanGckf/f and TanGck−/− mice four weeks after the injection (3 cohorts, n = 23/27, respectively). F–I. Representative images of epididymal white adipose tissue (eWAT, F-G) and subscapular brown adipose tissue (BAT, H–I) in adult male TanGckf/f (F, H) and TanGck−/− (G, I) mice. J-K. Quantification of mean area (J) and mean perimeter (K) of eWAT adipocytes in adult male TanGckf/f and TanGck−/− mice (n = 3/3). L. Quantification of BAT lipid droplet density in adult male TanGckf/f and TanGck−/− mice (n = 6/6). M-N. Gene expression in eWAT (M) and BAT (N) in a refed state in adult male TanGckf/f and TanGck−/− mice (n = 6/10, respectively). BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; FAO, fatty acid oxidation; GCK, glucokinase; W (in A-B), week. Scale bars: 100 μm in F–I. Data are means ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 compared to TanGckf/f group. Refer Supplementary Table 2.