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. 2021 Jul 26;53:101311. doi: 10.1016/j.molmet.2021.101311

Figure 8.

Figure 8

Tanycyte ablation induces a delay in meal termination after refeeding. A-B. Cumulative food intake (A) and food intake per period (B) in adult male TanGckf/f and TanGck−/− mice during 8-h refeeding after 24-h fasting (1 cohort, n = 8/7, respectively). C-D. Representative images of c-Fos activity in the ARH of adult male TanGckf/f and TanGck−/− mice after 2-h refeeding (asterisk = c-Fos activation in vmARH tanycytes). E-H. Quantification of the number of c-Fos-positive cells in the ARH (E), the vmARH (F), c-Fos-positive NPY neurons (G), and c-Fos-positive tanycytes along the vmARH (H) in adult male TanGckf/f and TanGck−/− mice after 2-h refeeding (n = 7/5, respectively). I-J. Gene expression of hypothalamic neuropeptides (I) and tanycytic markers (J) in microdissected hypothalami from adult male TanGckf/f and TanGck−/− mice after 2-h refeeding (n = 7/8 in fasted condition; n = 6/10 in refed condition, respectively). Agrp, agouti-related peptide; ARH, arcuate nucleus; Cart, Cocaine- and amphetamine-regulated transcript; Dio2, Iodothyronine Deiodinase 2; Fgf10, Fibroblast Growth Factor 10; GCK, glucokinase; Npy, Neuropeptide Y; Pomc, Pro-opiomelanocortin; Slc16a7, Monocarboxylate Transporter 2; 3v, third ventricle. Scale bars: 100 μm in C-D. Data are means ± SEM. ∗p < 0.05; ∗∗p < 0.01 compared to TanGckf/f group within the same metabolic condition. Refer to Supplementary Figure 7 and Supplementary Table 2.