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. 2021 Aug 8;46:102095. doi: 10.1016/j.redox.2021.102095

Fig. 3.

Fig. 3

IGF-II modulation of NRF2 and mTOR intracellular signalling pathways in SN4741 cells after MPP+-induced toxicity in cell cultures. (A) Experimental design for the study of the neuroprotective effects of IGF-II on NRF2 and mTOR intracellular signalling pathways. The measures were made in SN4741 cells after 2.5 h of incubation with MPP+, in the presence or absence of IGF-II and/or BMS and AB. (B) Representative immunocytochemistry stain for DAPI and NRF2 (C) Quantification of NRF2 immunofluorescence; BMS is used to define the receptor involved in the IGF-II effect (D) NRF2 target gene GST activity; AB is used to define the receptor involved in the IGF-II effect. (E) NRF2 target gene NQO1 activity; AB is used to define the receptor involved in the IGF-II effect. (F) Representative immunocytochemistry stain for DAPI and pmTOR (G) Quantification of pmTOR immunofluorescence; AB is used to define the receptor involved in the IGF-II effect. Data are expressed as mean ± SEM. n = 6 each group (3 independent experiment). #P < 0.05 versus CO and IGF-II groups; *P < 0.05, versus groups connected in bars; & P < 0.05, versus all other groups. Data were analysed by one-way ANOVA followed by Tukey's multiple comparison test.