Fig. 5.

Chronic IGF-II administration protects against behavioural deficits and dopaminergic degeneration in the MPTP/p mouse model of progressive PD. (A) Experimental design. (B) Motor deficits. (C) Self-grooming deficits. (D) TH-producing dopaminergic cells in SNc. The average coefficient of error (CE) of stereological counting is shown for each group. (E) TH expression in the striatum (Str). (F) DAT expression in the striatum. (G) GFAP⁺ cells in SNc. (H) GFAP⁺ cells in Str. (I) Representative immunostaining in every experimental condition. When applicable, black arrows indicate examples of positive immunolabeling. Data are expressed as mean ± SEM. *P < 0.05, difference between groups; #P < 0.05, versus control groups; & P < 0.05, versus all other groups; Ø P < 0.05, versus basal. Data were analysed by one-way ANOVA (immunohistochemical markers) or two-way ANOVA with repeated measures (rota-rod test and self-grooming) followed by Duncan's multiple range test.