Table 2.
Settings for imaging
| GFP excitation/emission | RFP excitation/emission | Laser power | Exposure time | EM gain | Z-stack | Imaging duration | Time interval |
|---|---|---|---|---|---|---|---|
| 488/505 nm | 561/595 nm | 3–5% | 30–100 ms | 200–300 | 11 sections with 1.0 μm spacing | 60–90 min | 1 or 1.5 min |