Subject	Bioinformatics/Omics: Transcriptomics
Specific subject area	Assembly, annotation and structural analysis of trypsin-like SerP and SerPH sequences from the Tenebrio molitor transcriptome using bioinformatics tools.
Type of data	Tables and Supplementary text file, containing assembled sequences in fasta format.
How data were acquired	RNA sequencing by Illumina HiSeq 2000, structural analysis with appropriate software
Data format	Raw
Parameters for data collection	Laboratory colonies of T. molitor were maintained on a diet consisting of either 50–100% oat flakes or 85% stabilized wheat germ. RNA was isolated and sequenced from the larval midgut and from eggs, I and IV instar larvae, pupae, young males and females with non-chitinized integuments and two week old adult males and females of T. molitor. Parameters for sequencing, contigs assembly and analysis programs were default.
Description of data collection	mRNA-isolation, high throughput RNA-sequencing using Illumina HiSeq 2000, automated and manual assembly of contigs to full-length sequences, annotation and structural analysis using bioinformatic tools.
Data source location	Institution: USDA Agricultural Research Service, Center for Grain and Animal Health Research City/Town/Region: Manhattan, KS 66502 Country: USA Latitude and longitude: 27.2046° N, 77.4977° E Institution: Lomonosov Moscow State University, A.N. Belozersky Institute of Physico-Chemical Biology City/Town/Region: Moscow, 119992 Country: Russia Latitude and longitude: 55.705836° N, 37.521456° E
Data accessibility	Repository name: NCBI Data identification number: Accession numbers unique to each sequence are listed in Tables 1 and 2. Direct URL to data: Direct URLs to data are presented as hyperlinks in Tables 1 and 2, which can be opened by clicking on the corresponding accession number. Data are also available with the article in the Supplementary file.