Table 4.
Comparison of cfa-miR-1841 expression by NGS and Q-RT-PCR. The values in the NGS data is the expression fold difference in comparison to NCF. The Q-RT-PCR values depicted are the Ct values from PCR amplification.
| 17–71 | OSW | BR | MPT-1 | DH82 | CF11 | D17 | COSC6 | COSC3 | CML10 | CML7 | CMT12 | CMT28 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NGS | cfa-miR-1841 | 96 | 46 | 2.4 | 8.1 | 46 | 6.4 | 3.7 | 27 | 6.0 | 41 | 10 | 129 | 72 |
| qPCR | cfa-miR-1841 | 27.3 | 29.9 | NA | NA | 26.3 | NA | 32 | NA | NA | 27.9 | NA | NA | NA |