Table 2.
Primer sequences used in the real-time PCR.
| Gene | Sequence (5′–3′) | GenBank accession number |
|---|---|---|
| AMPKα1 | F：GACTGCTACTCCACAGAGATCG | NM006251 |
| R：TCAGCATCTGAATCACTCCTTT | ||
| AMPKα2 | F：TGCGGAGGGCCATTCA | NM023991 |
| R：TGAGACAGAGGACGACATGCT | ||
| SIRT1 | F：CGAGAGGCGAGGAGGAGAAG | XM_015104377.1 |
| R：ATCGTTCGAGGATCTGTGCC | ||
| SIRT3 | F: GCCCAATGCCACTCACTACT | XM_004019744.3 |
| R: GGGATGCCAGATGCTCTCTC | ||
| PGC1α | F：CACCCACAACTCCTCCTCAT | AY321517.1 |
| R：CCTTCCTTTCCTCGTGTC | ||
| NRF1 | F：CGGAAGAGACAGCAAACACG | AY368269.1 |
| R：GGTTGGGTTTGGAGGGAGAG | ||
| NRF2 | F: CGAGCCGGTGTGAGTAGA | AY369137.1 |
| R: TTCCGTGGCCCAGTGTAAAG | ||
| ESRRA | F: TGCAGATCACCAAGCGAGAG | XM_015103167.1 |
| R: GTCTCACCTGTCTTCCGAGG | ||
| TFAM | F: CGCTCCCCCTTTAGTTTTGC | XM_015104510.1 |
| R: CTGCCAGTCTGCCCTGTAAG | ||
| mtD-loop | F: ACTCGGAGACCCAGACAACT | KY662385.1 |
| R: TGTAGGGGTGTTCAACTGGC | ||
| GAPDH | F：GTCAAGGCAGAGAACGGGAA | XM_012166462.1 |
| R：GGTTCACGCCCATCACAAAC |
AMPK = AMP-activated protein kinase; SIRT = silent information regulator; PGC1α = peroxisome proliferator-activated receptor γ coactivator-1α; NRF = nuclear respiratory factor; ESRRA = estrogen-related receptor alpha; TFAM = transcription factor A, mitochondrial; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.