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. 2021 Aug 21;20:164. doi: 10.1186/s12934-021-01654-8

Fig. 2.

Fig. 2

Selection of phosphinothrin-resistant transformants on a selective CYM plate. A Transformants on a selective CYM plate. 1, 2, 3: the strain transformed with pJW-EXP-opbar-flag; 4, 5, 6: the strain transformed with pJW-EXP-in-opbar-flag. B Identificaton of G. lucidum transformants by PCR. Lane M, DNA marker DL5000; Lane P, pJW-EXP-in-opbar-flag as positive control; Lane N, negative control; Lane 1, 2, 3, the transformants pJW-EXP-opbar-flag; Lane 4, 5, 6, the transformant pJW-EXP-in-opbar-flag