Table 2.

Metabolomics studies of microalgae: application of glycobiotechnology

Strain	Analytical method	Target product	Key findings	References
Chlorella vulgaris	MALDI-TOF MS, LC-ESI-MS	N-glycans	Analysis of N-glycosylation pathway in Chlorella vulgaris	[50]
Chlamydomonas reinhardti	MALDI-TOF-MS	N-glycans	Analysis of N-glycosylation Pathway in Chlamydomonas reinhardtii	[51]
Chlamydomonas reinhardtii	LC-ESI-MS	N-glycans	Reexamination of the N-glycosylation pathway in Chlamydomonas reinhardtii	[52]
Chlamydomonas reinhardtii	LC-ESI-MS	N-glycans	An additional xylosyl transferase is connected with the xylosylation of protein N-linked glycans	[53]
Phaeodactylum tricornutum	MALDI-TOF–MS	N-glycans, LLO	Study of lipid-linked oligosaccharides	[55]
Porphyridium sp.	MALDI-TOF MS, MS/MS	N-glycans	The structural analysis of the N-glycans in the 66-kDa glycoprotein	[54]
Phaeodactylum tricornutum	LC-ESI-MS	N-glycans	Turning out recombinant proteins using P. tricornutum with high mannose-type N-glycans condition	[56]
Phaeodactylum tricornutum	MALDI-TOF/TOF	N-glycans	Over expression of the FuT54599 leads to an increase of the α (1,3)-fucosylation of the diatom endogenous glycoproteins	[57]
Chlamydomonas reinhardtii	ESI-MS	O-glycans	Analysis of the O-glycosylation pathway in C. reinhardti	[58]
Chlamydomonas reinhardtii	MALDI-TOF-MS. Ma	O-glycans	Genes encoding the serine O-α-galactosyltransferase were separated from Chlamydomonas reinhardtii	[59]
Euglena gracilis	LC-ESI-MS	N-glycans, O-glycans	Euglena has a complex glycan surface	[63]
Chlorella vulgaris	GCMS, LC-MS/MS and MN (Molecular networking)	Metabolite of C.vulgaris using metabolomics including glycolipid	Finding the presence of glycolipid via novel bioinformatics approaches such as the molecular networking	[60]
Scytonema sp. UIC 10,036, and Nostoc sp. UIC 10,110	LC-HRMS, XCMS Online	Primary metabolites (heterocyst glycolipids)	Lower nitrate levels inducing increased amounts of heterocyst glycolipids	[62]
Chlorella UMACC050	LC-MS analysis	Lipid (including glycolipid)	Lipid accumulation including glycolipid and enhancement of glycolytic activity induced by nitrogen depletion	[64]