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. 2021 Aug 12;118(33):e2108963118. doi: 10.1073/pnas.2108963118

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Fig. 3. — LmjFUT1 is localized to the parasite mitochondrion. (A) Indirect immunofluorescence of parasites lines of Δfut1⁻/+pIR1NEO-FUT1-HA incubated with rabbit anti-HA antibody and visualized with goat anti-rabbit Alex 488-conjugated antibody (leftmost image, green), or MitoTracker Red CMXRos (central image, red), and merged (rightmost image, yellow) are shown. Colocalization by Pearson’s correlation coefficient was 0.97. (B) Cryo-immuno-EM of Δfut1⁻/+pIR1NEO-FUT1-HA. FUT1-HA was visualized using rabbit anti-HA followed by gold-bead–conjugated mouse anti-rabbit IgG. F, flagellar pocket; K, kinetoplast DNA network; M, mitochondrion. Controls in which the primary anti-HA antibody was omitted yielded no bead counts. (C) Quantitation of cryo-immuno-EM anti-HA bead labeling to FUT-HA in cellular compartments. The data shown are the bead counts and SD, taken from three different experiments comprising three sets of 10 sections each, and normalized to the relative compartment area.