Fig. 2.

SNI1 corresponds to rQTL4. (A) 420 crossover frequency for F₁ plants obtained from the cross between mutant lines for the six genes and F₆ individuals fixed for Col/Col and Ler/Ler over the rQTL4 interval. Measurements for the wild-type F₆ individuals for Col/Col, Col/Ler, and Ler/Ler were used as a control. Each dot represents one individual in A, C, D, and E. Significance in A, C, and D was assessed by Welch t test. (B) The genes within the rQTL4 19.5-kb Col/Ler segregating interval with non-synonymous substitutions indicated. (C) 420 crossover frequency for the mutants of genes located within rQTL4 locus. (D) Complementation of the sni1 mutation with SNI1^Col and SNI1^Ler alleles restores wild-type 420 crossover frequency in T₁ generation. (E) F₆ lines carrying SNI1^Ler are less responsive to temperature stress than the SNI1^Col as measured in the 420 interval. (G) Location of all non-synonymous substitution sites in the SNI1 gene observed within 1,135 A. thaliana accessions (46). The two substitutions differing Col and Ler alleles are indicated in red. (F) Phylogenetic tree of SNI1 orthologs identified by basic local alignment search tool (BLAST) searches in selected eukaryotic genomes. The phylogenetic tree was constructed using maximum likelihood method.