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. 2021 Aug 10;118(33):e2107095118. doi: 10.1073/pnas.2107095118

Fig. 2.

Fig. 2.

Experimental strategy to obtain solubilized MscS in open and closed conformations. MscS solubilized in DDM produced a closed conformation (condition 1), while washing with higher DDM concentrations during the purification led to an open conformation (condition 2a). Addition of lipids back to the same sample again produced a closed MscS (condition 3a). Exchange of DDM to LMNG during purification resulted in an open MscS (condition 2b), while addition of lipids partially closed MscS (condition 3b). Percentages refer to the number of particles in side-view 2D classes in the open or closed conformation relative to the total number of particles. The ratio of these percentages is an indication for the conformational distribution in the sample. MscS structures are shown in surface representation with different colors for the subunits. Examples of 2D class images are shown for all conditions in which the edge has a length of 27.2 nm.