Table 1.

Quantitation of embryo viability, male frequency, and diakinesis karyotypes

						Average DAPI bodies/nucleus ± SD (no. of oocytes)
Strain	Genotype	Incubated temperature, °C	Average brood size ± SD (no. of broods)	Dead eggs, %	Males, %	24 h after L4	48 h after L4
N2	WT	20	264 ± 27 (7)	0.4	0	6.0 ± 0.2 (92)	5.9 ± 0.3 (102)
N2	WT	25	291 ± 22 (7)	2	0	5.9 ± 0.3 (110)	5.9 ± 0.4 (99)
AV913	dsb-3(me6ts) IV	20	274 ± 27 (7)	0.1	0	6.0 ± 0.2 (144)	5.9 ± 0.3 (111)
AV913	dsb-3(me6ts) IV	25	198 ± 47 (7)	28.2	17.3	7.0 ± 1.4 (108)	9.9 ± 1.3 (99)
AV1095	dsb-3(me115) IV	20	156 ± 77 (16)	99.4	24.9	11.6 ± 0.6 (150)
AV1029	meSi7[sun1p::dsb-3::gfp::sun1 3′UTR unc-119+] II; dsb-3(me115) IV	20	150 ± 23 (8)	5	3.7	6.1 ± 0.4 (207)
AV1102	dsb-3(me125)[3xflag::dsb-3] dsb-1(me124)[3xha::dsb-1] IV	20	243 ± 34 (7)	2.1	2.5	6.0 ± 0.2 (213)
AV1115	dsb-2(me132)[3xha::dsb-2] II	20	193 ± 61 (8)	2.2	0.5	6.0 ± 0.2 (150)

Strains used in and/or created for this study were evaluated for the indicated parameters. Numbers of DAPI bodies were evaluated for worms fixed and stained at 24 h after L4 stage. Analysis of AV913 and corresponding controls were conducted at two temperatures based on the temperature-sensitive nature of the dsb-3(me6ts) mutant. UTR, untranslated region; WT, wild type.