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. 2021 Aug 12;118(33):e2012881118. doi: 10.1073/pnas.2012881118

Fig. 5.

Fig. 5.

The effects of circURI1 and hnRNPM on GC cell migration and invasion. (A) RT-qPCR analysis of circURI1 expression levels in WT or circURI1 KO AGS cells with ectopic circURI1 overexpression (OE) and circURI1 with the 19-nt mutation (circURI1-M19). (B and C) Wound-healing and Transwell assays in WT or circURI1 KO AGS cells with overexpressing circURI1 or circURI1-M19. (Scale bars, 100 μm.) (D) RT-qPCR analysis of hnRNPM expression levels in WT and circURI1 KO cells. (E) RT-qPCR analysis of knockdown efficiency of hnRNPM and circURI1 expression levels in AGS cells treated with two siRNAs against hnRNPM. siNC, siRNA with scrambled sequences; siM-1 and siM-2, two independent siRNAs against hnRNPM. (F and G) Wound-healing and Transwell assays of AGS cells transfected with full-length hnRNPM (hnRNPM [FL]) or co-overexpressed with circURI1 and circURI1-M19. (Scale bars, 100 μm.) (H and I) Wound-healing and Transwell assays of AGS cells overexpressing circURI1 or co-overexpressing hnRNPM truncated forms including hnRNPM (FL) and hnRNPM without the RRM1 domain (hnRNPM [Del_71-149aa]). (Scale bars, 100 μm.) Error bars indicate SEM from three independent experiments. N.S., not significant; *P < 0.05; **P < 0.01 by two-tailed Student’s t test.